BROWNING PREVENTION METHOD IN KEPEL (STELECHOCARPUS BURAHOL (BLUME) HOOK.F. & THOMSON) IN VITRO CULTURE

Ratih Restiani and Aniek Prasetyaningsih and Devi Sonti Sibarani (2024) BROWNING PREVENTION METHOD IN KEPEL (STELECHOCARPUS BURAHOL (BLUME) HOOK.F. & THOMSON) IN VITRO CULTURE. Jurnal Pembelajaran Dan Biologi Nukleus, 10 (1). pp. 243-253. ISSN 2685-7332

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Official URL: https://doi.org/10.36987/jpbn.v10i1.5464

Abstract

Browning of explants is a common problem in Kepel (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low regeneration of Kepelex plants. Using nodal explants, the effect of explant immersion in ascorbic acid, the addition of ascorbic acid to the media, as well asdark and light incubation conditions were investigated. The browning prevention method was selected based on the delayed browning appearance time, the lowest browning intensity, and the highest percentage of callus. This present research used a completely randomized design with treatment variations: immersion explant in ascorbic acid 100 mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel nodalex plants during 28 days of culture. The results of this research are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation.

Item Type:	Article
Uncontrolled Keywords:	Ascorbic acid; Activated charcoal; Browning; In vitro culture; Stelechocarpus burahol
Subjects:	S Pertanian > Pertanian (umum)
Divisions:	Fakultas Bioteknologi
Depositing User:	Beatrix Stefany
Date Deposited:	24 Sep 2024 02:43
Last Modified:	24 Sep 2024 02:43
URI:	http://katalog.ukdw.ac.id/id/eprint/9390

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