EFFECT OF METHYL JASMONATE ON BIOMASS AND SAPONIN CONTENT IN JAVANESE GINSENG (TALINUM PANICULATUM (JACQ.) GAERTN.) CALLUS CULTURE

Ratih Restiani and Dwi Aditiyarini and Novianti Barlin (2022) EFFECT OF METHYL JASMONATE ON BIOMASS AND SAPONIN CONTENT IN JAVANESE GINSENG (TALINUM PANICULATUM (JACQ.) GAERTN.) CALLUS CULTURE. Scholars Academic Journal of Biosciences, 10 (7). pp. 154-158. ISSN 2321-6883

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Official URL: https://doi.org/10.36347/sajb.2022.v10i07.003

Abstract

Talinum paniculatum (Jacq.) Gaertn, or known as Javanese Ginseng, refers to one of the medicinal plants that is often used as a raw material for herbal medicine. Its potential is inseparable from the bioactive compounds contained in every part of the plant, especially the saponin. One of the efforts that can be made to increase the saponin content is through the elicitation of callus culture using methyl jasmonate. Therefore, this study aimed to identify the effect of concentration and elicitation time of methyl jasmonate on biomass and saponin production in callus culture. The results showed that variations in concentration and elicitation time had no effect on the decrease of callus biomass when compared to control. The highest callus biomass was produced in the callus which was elicited in MS medium with the addition of a methyl jasmonate concentration of 0.05 mM for 5 days and 0.2 mM for 15 days. In addition, the largest saponin content by stain area (0.563 cm2 ) and the intensity of the TLC colour intensity (5 out of 5) on the callus were elicited at a methyl jasmonate concentration of 0.15 mM for 15 days. The results of this study indicated that the addition of methyl jasmonate to the callus culture medium could affect the saponin production. In addition, optimal concentration and elicitation time can increase biomass and saponin production in Javanese ginseng callus (T. paniculatum).

Item Type:	Article
Uncontrolled Keywords:	Talinum paniculatum, callus culture, elicitation, methyl jasmonate, saponin
Subjects:	Q Ilmu Pengetahuan > QH Sejarah Alam > QH301 Biologi
Divisions:	Fakultas Bioteknologi
Depositing User:	Beatrix Stefany
Date Deposited:	19 Sep 2024 06:55
Last Modified:	19 Sep 2024 06:55
URI:	http://katalog.ukdw.ac.id/id/eprint/9296

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