PROTECTIVE ROLE EFFECT OF MANNITOL AND METHYLPREDNISOLONE ON TESTICULAR INJURY POST TORSION-DETORSION IN RAT

Leonardo Cahyo Nugroho (2023) PROTECTIVE ROLE EFFECT OF MANNITOL AND METHYLPREDNISOLONE ON TESTICULAR INJURY POST TORSION-DETORSION IN RAT. International Journal of Pediatric Surgery, 4. ISSN 2766-5577

Text (Artikel Jurnal) Protective Role Effect of Mannitol and Methylprednisolone.pdf - Published Version Download (1MB)

Abstract

Introduction: Testicular torsion is one of the differential diagnoses of acute scrotal and is a urological emergency. When torsion occurs, the testicles experience ischemia, if blood flow is not restored, the damage will be irreversible starting from 4 hours and complete damage will occur at 8 hours to 12 hours after torsion. Methods: The samples in this study were 30 male Wistar rats aged 14-18 weeks with an average weight of 180 gm-230 gm, involving 2 large groups consisting of one control group and four intervention groups. Results: In the treatment group given both Mannitol and Methylprednisolone (group X1), there were significant decrease in MDA and Caspase-3 expression and significant increase in neutrophil adhesion. There was no significant different between group given Mannitol and/or Methylprednisolone and group underwent torsion-detorsion. Group X1 also showed the best results compared to other treatment groups regarding spermatogenesis and number of seminiferous tubular cell layer. Discussion: The osmotic properties of Mannitol provide a protective effect against I/R injury by restoring microvascular circulation in the early phase of the I/R injury period. Methylprednisolone can reduce neutrophil migration which in turn can reduce ROS and ultimately reduce apoptosis in germ cells. Conclusion: Antioxidant-antioxidant combinations are more effective than the use of a single antioxidant to reduce the occurrence of apoptosis and prevent reperfusion ischemic injury

Item Type:	Article
Uncontrolled Keywords:	Methylprednisolone; Mannitol; Torsion-detorsion; Diagnosis; Surgical intervention
Subjects:	Q Ilmu Pengetahuan > QL Zoologi / Ilmu Hewan
Divisions:	Fakultas Kedokteran
Depositing User:	Admin Repository
Date Deposited:	11 Sep 2024 03:18
Last Modified:	11 Sep 2024 03:18
URI:	http://katalog.ukdw.ac.id/id/eprint/9177

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