eprintid: 9390
rev_number: 11
eprint_status: archive
userid: 2098
dir: disk0/00/00/93/90
datestamp: 2024-09-24 02:43:00
lastmod: 2024-09-24 02:43:00
status_changed: 2024-09-24 02:43:00
type: article
metadata_visibility: show
contact_email: repository@staff.ukdw.ac.id
creators_name: , Ratih Restiani
creators_name: , Aniek Prasetyaningsih
creators_name: , Devi Sonti Sibarani
creators_id: 0512038702
creators_id: 0518096402
creators_id: 31190327
title: BROWNING PREVENTION METHOD IN KEPEL (STELECHOCARPUS BURAHOL (BLUME) HOOK.F. & THOMSON) IN VITRO CULTURE
ispublished: pub
subjects: S1
divisions: fak_biot
full_text_status: public
keywords: Ascorbic acid; Activated charcoal; Browning; In vitro culture; Stelechocarpus burahol
abstract: Browning  of  explants  is  a  common  problem  in  Kepel  (Stelechocarpus burahol (Blume) Hook.f. & Thomson) in vitro culture, resulting in low regeneration of Kepelex plants. Using nodal explants,  the  effect  of  explant  immersion  in  ascorbic  acid,  the  addition  of  ascorbic  acid  to  the media, as  well  asdark  and  light  incubation  conditions  were  investigated.  The  browning prevention  method  was  selected  based  on  the  delayed  browning  appearance  time,  the  lowest browning intensity, and the highest percentage of callus. This present research used a completely randomized  design  with  treatment  variations:  immersion  explant  in  ascorbic  acid  100  mg/L, addition of ascorbic acid 100 and 200 mg/L to the media, addition of ascorbic acid 100 and 200 mg/L and activated charcoal 1 g/L to the media, and incubation in dark and light conditions for 28 days. The results showed that the combination of the addition of ascorbic acid 200 mg/L to MS media and incubation in dark conditions were effective browning prevention methods in inhibiting browning appearance time (5 DAP), significantly reducing browning intensity (0.3), and increasing callus growth (100%) of kepel nodalex plants during 28 days of culture. The results of this research are useful in establishing protocols for in vitro culture of Kepel plants, especially at the initiation stage, and can be expected to support the successful conservation of Kepel plants through in vitro propagation.
date: 2024-03
publication: Jurnal Pembelajaran Dan Biologi Nukleus
volume: 10
number: 1
publisher: LPPM Universitas Labuhanbatu
pagerange: 243-253
id_number: doi:10.36987/jpbn.v10i1.5464
refereed: TRUE
issn: 2685-7332
official_url: https://doi.org/10.36987/jpbn.v10i1.5464
funders: ratih.restiani@staff.ukdw.ac.id
citation:   Ratih Restiani and Aniek Prasetyaningsih and Devi Sonti Sibarani  (2024) BROWNING PREVENTION METHOD IN KEPEL (STELECHOCARPUS BURAHOL (BLUME) HOOK.F. & THOMSON) IN VITRO CULTURE.  Jurnal Pembelajaran Dan Biologi Nukleus, 10 (1).  pp. 243-253.  ISSN 2685-7332     
document_url: https://katalog.ukdw.ac.id/9390/1/Browning%20Prevention%20Method%20in%20Kepel.pdf