eprintid: 9293
rev_number: 7
eprint_status: archive
userid: 2098
dir: disk0/00/00/92/93
datestamp: 2024-09-19 06:49:05
lastmod: 2024-09-19 06:49:05
status_changed: 2024-09-19 06:49:05
type: article
metadata_visibility: show
contact_email: repository@staff.ukdw.ac.id
creators_name: , Kukuh Madyaningrana
creators_name: , Stephan Immenschuh
creators_id: 0527068001
title: DETERMINATION OF FREE HEME IN STORED RED BLOOD CELLS WITH AN APO-HORSERADISH PEROXIDASE-BASED ASSAY
ispublished: pub
subjects: QH301
divisions: fak_biot
full_text_status: public
keywords: apoHRP assay; free heme; red blood cells; storage lesion; transfusion
abstract: Transfusion effectiveness of red blood cells (RBCs) has been associated with duration of the storage period. Storage-dependent RBC alterations lead to hemolysis and release of toxic free heme, but the increase of free heme levels over time is largely unknown. In the current study, an apo-horseradish peroxidase (apoHRP)-based assay was applied to measure levels of free heme at regular intervals or periodically in supernatants of RBCs until a maximum storage period of 42 days. Free heme levels increased with linear time-dependent kinetics up to day 21 and accelerated disproportionally after day 28 until day 42, as determined with the apoHRP assay. Individual time courses of free heme in different RBC units exhibited high variability. Notably, levels of free hemoglobin, an established indicator of RBC damage, and those of total heme increased with continuous time-dependent linear kinetics over the entire 42 day storage period, respectively. Supernatants from RBC units with high levels of free heme led to inflammatory activation of human neutrophils. In conclusion, determining free heme in stored RBCs with the applied apoHRP assay may become feasible for testing of RBC storage quality in clinical transfusion medicine.
date: 2022-09-05
publication: Journal Biological Chemistry
volume: 403
number: 11-12
publisher: De Gruyter
pagerange: 1091-1098
id_number: doi:10.1515/hsz-2022-0184
refereed: TRUE
official_url: https://doi.org/10.1515/hsz-2022-0184
funders: immenschuh.stephan@mh-hannover.de
citation:   Kukuh Madyaningrana and Stephan Immenschuh  (2022) DETERMINATION OF FREE HEME IN STORED RED BLOOD CELLS WITH AN APO-HORSERADISH PEROXIDASE-BASED ASSAY.  Journal Biological Chemistry, 403 (11-12).  pp. 1091-1098.      
document_url: https://katalog.ukdw.ac.id/9293/1/Determination%20of%20free%20heme.pdf