eprintid: 9204
rev_number: 13
eprint_status: archive
userid: 2098
dir: disk0/00/00/92/04
datestamp: 2024-09-13 03:14:14
lastmod: 2024-09-13 03:14:14
status_changed: 2024-09-13 03:14:14
type: article
metadata_visibility: show
contact_email: repository@staff.ukdw.ac.id
creators_name: , Naomi Bastanta Putri Octaviana Ginting
creators_name: , Ratih Restiani
creators_name: , Aniek Prasetyaningsih
creators_id: 31190273
creators_id: 0512038702
creators_id: 0518096402
title: EFFECT OF ASCORBIC ACID, ACTIVATED CHARCOAL AND DARK INCUBATION ON BROWNING INTENSITY OF SAURAUIA BRACTEOSA IN VITRO CULTURE
ispublished: pub
subjects: SB
divisions: fak_biot
full_text_status: public
keywords: Ascorbic acid; active charcoal; browning; in vitro culture; Saurauia bracteosa
abstract: Saurauia bracteosa, also known as Pirdot, is an endemic woody plant of the Actinidiaceae family with medicinal properties. Its population decline in nature has led to a vulnerable status, prompting conservation efforts, such as in vitro culture. Browning poses a significant challenge in the woody plant in vitro culture. Browning inhibitors such as ascorbic acid, activated charcoal and dark incubation can be used to address this issue. This study aims to determine the effect of ascorbic acid, activated charcoal and incubation conditions on browning inhibition of S.bracteosa. The study employed a Completely Randomized Design with 12 treatments on leaf explants, repeated five times. Observations, including the time of browning formation, percentage of browning, intensity of browning and percentage of live explant were carried out for 30 days and analyzed using ANOVA followed by DMRT. The results showed that the addition of activated charcoal in MS media with dark incubation delayed the browning formation time (12.40 DAI) while the adding ascorbic acid in MS media and dark incubation resulted in the lowest browning intensity (0.22). These research findings can serve as a foundational protocol for browning prevention, supporting the successful in vitro conservation of S. bracteosa.
date: 2023-12-08
publication: Biosaintifika: Journal of Biology and Biology Education
volume: 15
number: 3
publisher: Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang Perhimpunan Biologi Indonesia in collaboration with Perhimpunan Biologi Indonesia Tingkat Pusat.
pagerange: 401-411
id_number: doi:10.15294/biosaintifika.v15i3.48439
refereed: TRUE
issn: 2338-7610
official_url: https://doi.org/10.15294/biosaintifika.v15i3.48439
funders: ratih.restiani@staff.ukdw.ac.id
citation:   Naomi Bastanta Putri Octaviana Ginting and Ratih Restiani and Aniek Prasetyaningsih  (2023) EFFECT OF ASCORBIC ACID, ACTIVATED CHARCOAL AND DARK INCUBATION ON BROWNING INTENSITY OF SAURAUIA BRACTEOSA IN VITRO CULTURE.  Biosaintifika: Journal of Biology and Biology Education, 15 (3).  pp. 401-411.  ISSN 2338-7610     
document_url: https://katalog.ukdw.ac.id/9204/1/Effect%20of%20Ascorbic%20Acid%2C%20Activated%20Charcoal%20and%20Dark%20Incubation.pdf