eprintid: 9203
rev_number: 11
eprint_status: archive
userid: 2098
dir: disk0/00/00/92/03
datestamp: 2024-09-13 03:13:29
lastmod: 2024-09-13 03:13:29
status_changed: 2024-09-13 03:13:29
type: article
metadata_visibility: show
contact_email: repository@staff.ukdw.ac.id
creators_name: , Rafaela Marlisa. G. M.
creators_name: , Ratih Restiani
creators_name: , Aniek Prasetyaningsih
creators_id: 31190281
creators_id: 0512038702
creators_id: 0518096402
title: EFFECT OF 2,4-D AND BAP ON THE SAURAUIA BRACTEOSA IN VITRO CULTURE
ispublished: pub
subjects: SB
divisions: fak_biot
full_text_status: public
keywords: Browning, in vitro culture, PGR, recalcitrant, Saurauia bracteosa
abstract: Saurauia bracteosa DC. (Pirdot) is one of the endemic woody plant species that has medicinal properties. The declining population in natural habitats caused this plant as a vulnerable species that need in vitro culture to prevent further extinction. The combination of 2.4-D and BAP as an exogenous PGR influenced the success of woody plant in vitro culture. Therefore, this research aimed to determine the effect of 2.4-D and BAP concentrations on the in vitro response of the S. bracteosa leaf explant. This research used a complete randomized design (CRD) with two factors: (1) the concentration of 2.4-D (0.50; 0.75; 1 mg/L), and (2) the concentration of BAP (B) (1; 2; 3 mg/L). This research consisted of 16 treatments (including a control); each treatment was repeated three times. All data were analyzed statistically using ANOVA, and the significant results will be followed by Duncan’s Multiple Range Test (DMRT). All PGR treatments could not produce a callus growth response, yet all explants showed various responses to browning intensity (0.08–1) and 100% browning. Further research still needs to be done to find the best type of explant and concentrations of PGR that can overcome the recalcitrant explant of Sauraia bracteosa. These results can be valuable information to promote the successful in vitro conservation of Sauraia bracteosa.
date: 2023-12-16
publication: Scholars Academic Journal of Biosciences
volume: 11
number: 12
publisher: SAS Publishers
pagerange: 461-466
id_number: doi:10.36347/sajb.2023.v11i12.007
refereed: TRUE
issn: 2321-6883
official_url: https://doi.org/10.36347/sajb.2023.v11i12.007
citation:   Rafaela Marlisa. G. M. and Ratih Restiani and Aniek Prasetyaningsih  (2023) EFFECT OF 2,4-D AND BAP ON THE SAURAUIA BRACTEOSA IN VITRO CULTURE.  Scholars Academic Journal of Biosciences, 11 (12).  pp. 461-466.  ISSN 2321-6883     
document_url: https://katalog.ukdw.ac.id/9203/1/Effect%20of%202%2C4-D%20and%20BAP.pdf