TY  - JOUR
ID  - katalog9131
PB  - medtextpublications.com
Y1  - 2023/02//
EP  - 9
TI  - PROTECTIVE ROLE EFFECT OF MANNITOL AND METHYLPREDNISOLONE ON TESTICULAR INJURY POST TORSION-DETORSION IN RAT
JF  - International Journal of Pediatric Surgery
UR  - https://www.medtextpublications.com/international-journal-of-pediatric-surgery-home.php
SN  - 2766-5577
SP  - 4
AV  - public
N2  - Introduction: Testicular torsion is one of the differential diagnoses of acute scrotal and is a urological emergency. When torsion occurs, the testicles experience ischemia, if blood flow is not restored, the damage will be irreversible starting from 4 hours and complete damage will occur at 8 hours to 12 hours after torsion. Methods: The samples in this study were 30 male Wistar rats aged 14-18 weeks with an average weight of 180 gm-230 gm, involving 2 large groups consisting of one control group and four intervention groups. Results: In the treatment group given both Mannitol and Methylprednisolone (group X1), there were significant decrease in MDA and Caspase-3 expression and significant increase in neutrophil adhesion. There was no significant different between group given Mannitol and/or Methylprednisolone and group underwent torsion-detorsion. Group X1 also showed the best results compared to other treatment groups regarding spermatogenesis and number of seminiferous tubular cell layer. Discussion: The osmotic properties of Mannitol provide a protective effect against I/R injury by restoring microvascular circulation in the early phase of the I/R injury period. Methylprednisolone can reduce neutrophil migration which in turn can reduce ROS and ultimately reduce apoptosis in germ cells.
Conclusion: Antioxidant-antioxidant combinations are more effective than the use of a single antioxidant to reduce the occurrence of apoptosis and prevent
reperfusion ischemic injury.
VL  - 4
A1  - Leonardo Cahyo Nugroho
A1  - Afif Iman Hidayat
A1  - Adityas Mahendra Putra
KW  - Methylprednisolone; Mannitol; Torsion-detorsion; Diagnosis; Surgical intervention
ER  -