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<http://katalog.ukdw.ac.id/id/eprint/1728> <http://purl.org/dc/terms/title> "ANALISIS FILOGENETIK ISOLAT Salmonella spp BERDASARKAN PENANDA GEN invA"^^<http://www.w3.org/2001/XMLSchema#string> .
<http://katalog.ukdw.ac.id/id/eprint/1728> <http://purl.org/ontology/bibo/abstract> "Gen invA merupakan gen yang sifatnya highly conserved pada genus Salmonella sehingga dapat digunakan untuk meneliti kekerabatan antar serovar yang memiliki fenotip patogen. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan Salmonella spp asal susu sapi dengan Salmonella patogen lain berdasarkan penanda gen invA. Isolat digunakan dalam penelitian ini adalah Salmonella spp PTKWM asal susu segar yang diisolasi dan diidentifikasi sebelumnya oleh Gadi (2010). Konfirmasi kemurnian isolat dilakukan dengan inokulasi isolat pada medium Rambach Agar dan Salmonella Shigella Agar. DNA diisolasi menggunakan kit isolasi DNA komersial, kemudian diamplifikasi menggunakan penanda invA. Produk PCR selanjutnya disekuensing dan dianalisis menggunakan piranti lunak bioinformatika untuk mengkonstruksi pohon filogenetik. Analisis filogenetik membuktikan bahwa isolat Salmonella spp memiliki hubungan kekerabatan dengan isolat standar Salmonella Typhi NTCT 786. Gen invA terbukti dapat digunakan sebagai penanda kekerabatan antar spesies Salmonella."^^<http://www.w3.org/2001/XMLSchema#string> .
<http://katalog.ukdw.ac.id/id/eprint/1728> <http://purl.org/dc/terms/date> "2016-11" .
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